Metagenomics Workflow

Metagenomics is revolutionizing how we diagnose and understand infectious diseases. Unlike traditional tests that look for a single pathogen, metagenomics analyzes all genetic material in a sample β€” including bacteria, viruses, fungi, and parasites β€” to give a complete microbial profile.

Why Metagenomics Matters

  • Comprehensive detection β€” finds both known and unknown pathogens
  • Unbiased & untargeted β€” no assumptions about what you're testing for
  • Co-infection detection β€” sees the whole picture, not just one microbe
  • Quantitative data β€” get a sense of abundance and balance
  • One test, many answers β€” reduce time, cost, and diagnostic error

Metagenomics vs. Traditional Methods

Ova & Parasite (O&P) Exam

  • O&P: Relies on visual identification under a microscope
  • Metagenomics: Detects DNA/RNA from parasites missed by microscopy
  • Detects low-burden or early-stage infections
  • Identifies multiple parasites in a single test
  • Can detect all life stages β€” eggs, larvae, cysts

PCR (Polymerase Chain Reaction)

  • PCR: Targets specific, known sequences
  • Metagenomics: Reads everything in the sample
  • No prior guesswork needed β€” detects unexpected or novel organisms
  • Detects co-infections & microbial ecosystem interactions
  • Screens for antimicrobial resistance genes

Real-World Applications

  • Clinical Diagnostics: Solves mystery illnesses where standard tests fail
  • Outbreak Tracking: Identifies new pathogens and variants
  • Microbiome Research: Reveals links between microbes, immunity, and chronic disease
  • Environmental Surveillance: Monitors microbial diversity in soil, water, sewage
  • Public Health: Tracks antibiotic resistance across populations

At PathoID.AI, we combine metagenomic sequencing with advanced AI to uncover infections others miss. It’s not just a test β€” it’s a window into your body’s microbial truth.

Uncover the Invisible
One Sequence at a Time